Scholar Hub Community:
https://yscholarhub.yonsei.ac.kr/handle/2021.sw.yonsei/4817
2024-03-29T11:31:49Z
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Changes in DNA methylation after 6-week exercise training in colorectal cancer survivors: A preliminary study
https://yscholarhub.yonsei.ac.kr/handle/2021.sw.yonsei/6422
Title: Changes in DNA methylation after 6-week exercise training in colorectal cancer survivors: A preliminary study
Authors: Seo-Hyeon Hwang; Dong-Woo Kang; Mi-Kyung Lee; Ji Yong Byeon; Hanui Park; Dong-Hyuk Park; Kyung-Chul Kim; SEUNG-TAE LEE; SangHui Chu; NAM KYU KIM; JUSTIN Y JEON
Abstract: Aim: Behavioral interventions such as exercise may induce epigenetic changes. Only few studies investigated the effects of exercise on epigenetic alterations in colorectal cancer survivors. The aim of this study was to explore the changes of genome-wide DNA methylation after 6-week exercise training in colorectal cancer survivors. Methods: This preliminary study used a subset of data from a randomized controlled trial in 15 colorectal cancer survivors. Participants were randomized either to the 6-week exercise group or control group. The exercise intervention consisted of a weekly, group-based, supervised resistance exercise program and a home-based same resistance exercise plus walking six times per week. Blood samples were collected at baseline and after the intervention and data from eight subjects were analyzed for genome-wide DNA methylation on 865,918 CpG sites. Results: Compared to the control group, the exercise group shows notable methylation changes in 756 CpG sites (22.7?25.2%). Gene ontology and disease annotation analysis showed that the genes targeting 81 CpG sites in promoter region with significant group-difference were linked in biological process such as immune response and transcription and related to metabolic and immune diseases. Also, hypermethylation on genes related to disease prevention seemed to be inhibited in the exercise group compared to the control group, indicating a likelihood of transcriptional activity of these genes. Conclusion: We found a preliminary evidence of the positive effects of exercise intervention on epigenetic markers in colorectal cancer survivors. Larger scale randomized controlled trials are warranted to further investigate our findings.
2022-02-01T00:00:00Z
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Profiling of conditionally reprogrammed cell lines for in vitro chemotherapy response prediction of pancreatic cancer
https://yscholarhub.yonsei.ac.kr/handle/2021.sw.yonsei/5256
Title: Profiling of conditionally reprogrammed cell lines for in vitro chemotherapy response prediction of pancreatic cancer
Authors: Hee Seung Lee; EunYoung Kim; JINYOUNG LEE; seongjoon park; ho kyoung hwang; CHANHEE PARK; 조세영; Chang Moo Kang; Seung-Mo Hong; Huapyong Kang; JUNG HYUN JO; INRAE CHO; Moon Jae Chung; JEONG YOUP PARK; Seung Woo Park; SI YOUNG SONG; Jung Min Han; SANGWOO KIM; Seungmin Bang
Abstract: Background: The establishment of patient-derived models for pancreatic ductal adenocarcinoma (PDAC) using conventional methods has been fraught with low success rate, mainly because of the small number of tumour cells and dense fibrotic stroma. Here, we sought to establish patient-derived model of PDAC and perform genetic analysis with responses to anticancer drug by using the conditionally reprogrammed cell (CRC) methodology. Methods: We performed in vitro and in vivo tumourigenicity assays and analysed histological characteristics by immunostaining. We investigated genetic profiles including mutation patterns and copy number variations using targeted deep sequencing and copy-number analyses. We assessed the responses of cultured CRCs to the available clinical anticancer drugs based on patient responsiveness. Findings: We established a total of 28 CRCs from patients. Of the 28 samples, 27 showed KRAS mutations in codon 12/13 or codon 61. We found that somatic mutations were shared in the primary-CRC pairs and shared mutations included key oncogenic mutations such as KRAS (9 pairs), TP53 (8 pairs), and SMAD4 (3 pairs). Overall, CRCs preserved the genetic characteristics of primary tumours with high concordance, with additional confirmation of low-AF NPM1 mutation in CRC (35 shared mutations out of 36 total, concordance rate=97.2%). CRCs of the responder group were more sensitive to anticancer agents than those of the non-responder group (P < 0.001). Interpretation: These results show that a pancreatic cancer cell line model can be efficiently established using the CRC methodology, to better support a personalized therapeutic approach for pancreatic cancer patients. Funding: 2014R1A1A1006272, HI19C0642-060019, 2019R1A2C2008050, 2020R1A2C209958611, and 2020M3E5E204028211 © 2021 The Authors
2021-03-01T00:00:00Z
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Radiotherapy-induced high neutrophil-to-lymphocyte ratio is a negative prognostic factor in patients with breast cancer
https://yscholarhub.yonsei.ac.kr/handle/2021.sw.yonsei/6462
Title: Radiotherapy-induced high neutrophil-to-lymphocyte ratio is a negative prognostic factor in patients with breast cancer
Authors: Chang Ik Yoon; Dooreh Kim; SUNG GWE AHN; SOONGJUNE BAE; Cha Chihwan; So Eun Park; Seho Park; Seung Il Kim; HYUN-SUN LEE; JU YOUNG PARK; Jeong Joon
Abstract: Radiotherapy (RT) is the standard of care following breast-conserving operation in breast cancer patients. The neutrophil-to-lymphocyte ratio (NLR) reflects the systemic change caused as a result of the radiotherapy. We aimed to evaluate the association between RT and the change in NLR following the receipt of RT, and to investigate the prognostic impact. We retrospectively reviewed NLR values of breast cancer patients taken before the administration of the first and the last session of RT. The cut-off point for the NLR was determined using the Youden index and receiver operating characteristic (ROC) curve within the training set. Recurrence-free survival (RFS), distant metastasis free survival, and overall survival were the main outcomes. Patients with an NLR higher than 3.49 after RT were classified to an RT-induced high NLR group and showed a significantly higher recurrence rate compared to those with low NLR (p < 0.001). In a multivariate Cox proportional hazards model, RT-induced high NLR remained a significant prognostic factor (HR 2.194, 95% CI 1.230?3.912, p = 0.008 for tumor recurrence. We demonstrated that an increase in NLR over the course of RT has a negative impact on survival, putting these patients with RT-susceptible host immunity at a higher risk of tumor recurrence.
2020-07-01T00:00:00Z