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초록

This study examined the effect of the high permeate flux on continuous biohydrogen production using dynamic membrane bioreactor (DMBR). A lab-scale DMBR was equipped with polyester mesh having two pore sizes, 220 mu m and 444 mu m, to avoid washout and membrane fouling, respectively. The DMBR was continuously fed with 20 g glucose/L at a hydraulic retention time of 3 h, while the permeate flux increased from 116 to 291 L/m2.h over 58 days, under non-sterile conditions. The highest average hydrogen production rate, hydrogen yield, and daily hydrogen production were observed as 21.7 +/- 1.7 L H2/L/d, 1.41 +/- 0.05 mol H2/mol hexoseconsumed, and 184.0 +/- 9.3 L H2/d, respectively, at the permeate flux of 233 +/- 12 L/m2.h, with an average permeability for 444-mu m mesh was 25.42 L/m2.h/kPa at an average transmembrane pressure (TMP) of 9 +/- 1.2 kPa. Higher permeate flux (>= 291 +/- 14 L/m2.h) caused severe biomass washout, while lower permeate flux (<= 175 +/- 9 L/m2.h) resulted in excessive cake layer along with the increased H2 consuming pathways. At the optimum permeate condition, H2producing granules were formed, which were with an average size of 1875 mu m and a size distribution of 1150-2580 mu m for 60% of the volume fraction. Although the reactor was inoculated with Clostridium butyricum DSM 10702, the dominant microbial species became Clostridium pasteurianum and Ethanoligenens harbinense as it was operated under non-sterile conditions.