Investigation on achieving super-resolution by solid immersion lens based STED microscopy
- Authors
- Kim, Wan-Chin; Moon, Hyungbae; Lee, Won-Sup; Lim, Geon; Choi, Guk-Jong; Kang, Donyoung; Lee, Hyungsuk; Park, No-Cheol
- Issue Date
- Jul-2017
- Publisher
- OPTICAL SOC AMER
- Keywords
- STED
- Citation
- OPTICS EXPRESS, v.25, no.14, pp 16629 - 16642
- Pages
- 14
- Journal Title
- OPTICS EXPRESS
- Volume
- 25
- Number
- 14
- Start Page
- 16629
- End Page
- 16642
- URI
- https://yscholarhub.yonsei.ac.kr/handle/2021.sw.yonsei/6645
- DOI
- 10.1364/OE.25.016629
- ISSN
- 1094-4087
- Abstract
- The feasibility of stimulated emission depletion( STED) microscopy using a solid immersion lens was investigated. First, the theoretical feasibility of the considered system is discussed based on a vectorial field algorithm that uses a stratified medium composed of a SIL air-gap and test sample. Using the simulation, we verified that evanescent waves with much higher spatial frequencies corresponding to the high numerical aperture in the air-gap can be utilized to achieve a higher resolution than a confocal fluorescent image without a depletion beam. The simulated expectation was supported by actual imaging on two types of samples: fluorescent beads with a 20 nm diameter and an actin sample with a filamentous structure. The lateral resolution of the system was determined to be 34 nm via the imaging results on the nano-beads. The system was quite promising for achieving nano-scale surface imaging of biological samples; an even higher resolution was achieved by adjusting the wavelength and the intensity of the depletion beam. (C) 2017 Optical Society of America
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Collections - College of Engineering > Mechanical Engineering > 1. Journal Articles
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